| Knife type |
Knife angle |
Size
[mm] |
Thickness
range [nm] |
Boat type |
Code
(new knife)* |
Application |
| cryo immuno |
35° |
2.0mm
3.0mm |
30-300 |
Triangular holder |
DCIMM3520
DCIMM3530 |
Sucrose infiltrated samples (Tokuyasu)
Frozen hydrated samples (CEMOVIS)
|
The first cryo knife with a diamond platform, guarantees the best possible sectioning for sucrose infiltrated samples (Tokuyasu).
The diamond platform guarantees an easy and gentle section pick-up.
The sections are collected directly from the diamond surface using a loop and a sucrose/methyl-cellulose droplet (W. Liou et al., Histochemistry and Cell Biology, Vol. 106, pp. 41 – 55, 1996. P . J. Peters et al., Current Protocols in Cell Biology, pp. 4.7.1 – 4.7.18, 2006).
The 35° angle leads to a considerable reduction in mechanical stresses and therefore to improved structure preserva- tion in sucrose infiltrated samples (E. Bos et al., Journal of Structural Biology 175, pp. 62 – 72, 2011).
We recommend the cryo immuno knife also for sectioning frozen hydrated samples (CEMOVIS). The 35° angle is a good compromise between durability and cutting performance (A. Al-Amoudi et al., Journal of Structural Biology 150, pp. 109 – 121, 2005).
