cro immunoCryo Immuno Diamond Knife

cryo immuno

The first cryo knife with a diamond platform, guarantees the best possible sectioning for cryo-immuno-chemistry.

The diamond platform guarantees easy and gentle section collection. Sections are collected directly from the diamond surface using a loop and a sucrose/methylcellulose droplet (Refs. Liou, Peters).

The 35° cutting angle leads to a considerable reduction in mechanical stresses and therefore to improved structure preservation in sucrose-protected samples.

Specifications

Knife angle: 35°
Cutting range: 30nm - 1μm
Available sizes: 2mm, 3mm

immuno diamond knife
Fusion of late endosome/lyosome with the Mycobacteria tuberculosis phagosome. Aldehyde fixed cryo-section labeled with anti- CD63 and protein-A gold 10nm. Scale bar = 100nm. Nicole van der Wel and Peter J. Peters, Netherlands Cancer Institute, Amsterdam.

References

A. Al-Amoudi, D. Studer and J. Dubochet: Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy. Journal of Structural Biology 150, pp. 109-121, 2005.

W. Liu, H. J. Geuze, J. W. Slot: Improving structural integrity of cryosections for immunogold labeling Histochemistry and Cell Biohgy, Vol. 106, PP. 41-55, 1996.

M. Michel, H. Gnagi and M. Muller: Diamonds are a cryosectioner's best friend. Journal of Microscopy, Vol 166, Pt 1, 43-56, 1992.

P. J. Peters: Cryo-lmmunogold Electron Microscopy, In Current Protocols in Cell Biology (J.S. Bonifacino, M. Dasso, J.B. Harford, J. Lippincott-Schwartz and K.M. Yamada, eds) pp. 4.7.1-4.7.12, 1999. Jonn Wiley & Sons, New York.

K. Richter: Cutting artifacts on ultrathin cryosections of biological bulk specimens. Micron, Vol. 25, No. 4, pp. 297-308. 1994.

P. Zhang, E. Bos, J. Heymann, H. Gnaegi, M. Kessel, P.J. Peters, S. Subramaniam: Direct visualisation of receptor arrays in frozen-hydrated sections and plungefrozen specimens of E.coli engineered to
overproduce the chemotaxis receptor Tsr. Journal of Microscopy, Vol. 216, Pt 1, pp. 76-83, 2004.

Ordering Information

All products can be ordered online using a credit card or purchase order number for payment. Order online:

Diamond Knives and accessories can be ordered by phone at 215-412-8390 or fax 215-412-8450.

 

Your benefits of working with Diatome

Free customer service

Sectioning tests with biological and material research specimens of all kinds.

We send you the sections along with the surfaced sample, a report on the results obtained and a recommendation of a suitable knife.

Complete discretion when working with proprietary samples.

Re-sharpening and reworking service

A re-sharpened Diatome diamond knife demonstrates the same high quality as a new knife.

Even knives purchased in previous years can continue to be re-sharpened.

All knives can be reworked into another type of knife for no extra charge, e.g. ultra to cryo or 45° to 35°.

Exchange service

Whenever you exchange a knife we offer you a new Diatome.

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Contact Us

1560 Industry Road
P.O. Box 410
Hatfield, PA 19440
tel: 215.412.8390
fax: 215.412.8450
email: sgkcck@aol.com

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