Peripheral nerve (rat), HP frozen, freeze substituted, Epon embedded, cut with the ultra sonic knife, section thickness 50nm. W. Graber, Institut of Anatomy, University of Bern, Switzerland
Heart (rat), fixed with 2.5% Glutaraldehyde, OsO4 stained, Spurr's embedded, cut with the ultra sonic knife, section thickness setting 15nm. J. Nebesarova, Institut of Parasitology, Ceske
Budejovice, Czech Republic. Imaged by Petr Stepan in LVEM 5 at 5kV, Delong Instruments, Brno, Czech Republic.
Human keratinocyte. HP frozen, substituted with methanol -0.5% uranyl acetate at -90°C, embedded in Lowicryl HM20 at -40° C. Cut with the ultra sonic knife, section thickness 50nm. The cell
nucleus (N) is surrounded by an area rich in tonofilaments (T). Several electron dense melanosomes (M) are apparent on the top left side of the cell. The peripheral area of the cell contains
numerous vesicles displaying a regular spherical appearance. Daničle Spehner, E0345 INSERM, EFS-Alsace, Strasbourg, France.
ABS, stained with OsO4, sectioned at room temperature with the ultra sonic knife, section thickness 50nm. Note the almost perfect spherical shape of the large rubber particles and the preservation
of the inclusions inside. Also the smaller dense rubber particles are well preserved. B.Vastenhout, Dow Benelux N.V. Terneuzen, The Netherlands.