1. |
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2. |
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Setting the clearance angle.
Mounting the cryotrim diamond blade and the knife.
Distance of electrode-knife approx. 25mm. |
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Tightening all set screws. |
3.
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Trimming with the cryotrim 45 diamond blade: ionizer on Pos. 10.
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4. |
Start sectioning with the cryo immuno knife: ionizer on Pos. 10, distance of electrode - knife approx. 10mm.
After 2 - 3 sections: distance of electrode approx. 25mm. |
4a. |
If sections lift up from the knife surface: reduce voltage. |
4b. |
If sections stick at the cutting edge: increase voltage. |
5.
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6. |
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Manipulating sections with an eyelash: ionizer on Pos. 2. |
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Use special care when picking-up the sections:
Do not touch the cutting edge with any solid objects. |
7. |
Remove knife from the chamber (before heating up!). |
 Cleaning
- Bevel one of our polystyrol rods to an angle of approx. 60°.
- Dip in ethanol 50% and shake off the excess.
Gently run the rod across the cutting edge without applying lateral pressure.
- If sections or debris dry on the knife edge:
Place the knife in distilled water overnight. Clean the knife as described above.
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