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2. |
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Pull the sections away from the cutting edge of the diamond knife with an eyelash. |
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Dip the LOOP in sucrose or sucrose/methyl-cellulose. |
3.
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4.
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Hold the LOOP above the cryo sections. |
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Touch the sections with the almost frozen sucrose or sucrose methyl-cellulose. Gently lift up the LOOP and bring back to room-temperature. |
5.
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6. |
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Lower the LOOP onto a grid and lift up again. |
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Place the LOOP upside-down on a water surface to wash out the sucrose or sucrose methyl-cellulose and allow the grid to float free. |