Perfect Loop for Cryo Sectioning

1. Perfect Loop for Cryo Sectioning 2. Perfect Loop for Cryo Sectioning
  Pull the sections away from the cutting edge of the diamond knife with an eyelash.   Dip the LOOP in sucrose or sucrose/methyl-cellulose.

3.

 

Perfect Loop for Cryo Sections

4.

 

Perfect Loop for Cryo Sections
  Hold the LOOP above the cryo sections.   Touch the sections with the almost frozen sucrose or sucrose methyl-cellulose. Gently lift up the LOOP and bring back to room-temperature.

5.

 

Perfect Loop for Cryo Sections 6. Perfect Loop for Cryo Sections
  Lower the LOOP onto a grid and lift up again.   Place the LOOP upside-down on a water surface to wash out the sucrose or sucrose methyl-cellulose and allow the grid to float free.

Please visit Electron Microscopy Sciences for additional information on the Perfect Loop.