histo Diamond Knives
DiATOME histo knives and their applications
- High quality diamonds guarantee perfect sections and a durable cutting edge
- Easy wetting cutting edge
- Optimised serial sectioning
- Large boat for easy pick-up
| Knife type | Knife angle | Size [mm] |
Thickness range [µm] |
Boat type | Code (new knife)* |
Application |
|---|---|---|---|---|---|---|
| histo | 45° | 4.0mm 6.0mm 8.0mm |
0.2-2 | histo boat | 40-HIS 60-HIS 80-HIS |
Sectioning biological and industrial materials specimens for optical microscopy |
| histo Jumbo | 45° | 6.0mm 8.0mm |
0.2-2 | Jumbo boat | 60-HISJ 80-HISJ |
Large boat for serial sectioning |
| histo cryo (dry) |
45° |
4.0mm |
0.2-2 | histo holder | 40-HDC 60-HDC 80-HDC |
Sectioning biological and materials specimens for optical microscopy |
| histo cryo (wet) |
45° | 4.0mm 6.0mm 8.0mm |
0.2-2 | Small histo boat | 40-HWC 60-HWC 80-HWC |
Wet sectioning of polymers (with DMSO/water mixture) |
* Product Codes shown are for new knives.
To order a resharpened knife, add “R” to the Product Code that corresponds to your knife.
Example: Product Code 40-HISR = Resharpened histo 4.0mm Knife.
Histo, histo Jumbo, and histo cryo can be exchanged. To order an exchanged knife, add “E” to the Product Code.
Example: Product Code 60-HISJE = Exchange of a histo Jumbo 6.0mm Knife.
histo
The histo knife is designed for the sectioning of hard and soft biological and material research specimens, non embedded or embedded in acrylic or epoxy resins (O. L. Reymond, Bas. Appl. Histochem. 30, pp. 487 – 494, 1986).
Our histo knives can be used on all ultramicrotomes as well as on microtomes with a retraction of the specimen arm in the return phase
Nondecalcifi ed rat bone. Scale: 35 mm = 100 μm. Daniel Studer, Anatomisches Institut, Bern.
histo cryo
Our histo cryo knives are delivered with a boat for wet sectioning using a DMSO/water mixture or in triangular holders for dry sectioning
E. B. Hunziker, M. E. Müller, Institute for Biomechanics, University of Bern. Rabbit joint, calcified cartilage/bone. X760
histo Jumbo
For 3D reconstruction it is imperative not to lose a single section (M. J. F. Blumer et al., Journal of Neuroscience Methods 120, pp. 11 – 16, 2002).
The large Jumbo boat as well as the adhesive (Pattex compact by Henkel) applied to the upper side of the sample block increase the distinct advantages of our histo knives.
They allow:
- easy production of section ribbons (0.5 – 2 μm)
- no section loss
- no folding
- the same orientation of all sections
- easy collection of section ribbons
- multiple ribbons on one glass slide
- perfect for immuno-histochemistry
Eye of A. peroni: photographic sequence of some semithin sections of a complete serie through the eye.
L = lens; RE = retina.
Michael J. F. Blumer, Institut für Zoologie, Universität Wien.
Reprinted from: Ribbons of semithin sections: an advanced method with a new type of diamond knife. Journal of Neuroscience Methods 120, pp. 11 – 16, 2002, with permission from Elsevier.
