DiATOME Knives and Blades

Development, Manufacturing and Customer Service since 1970

Diamond Blades & Knives

Diamond Blades & Knives

Diamond blades and diamond knives are essential microtomy tools utilized for semithin and ultrathin sectioning, sample preparation, and other application processes in fields such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), light microscopy, and Cryo-Electron Microscopy (cryo-EM). Each diamond knife is designed with unique properties that enable precision sectioning with minimal artifacts in application-specific environments. Explore the array of fields and use cases for diamond blades and microtomy below.

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Ultramicrotomy

Ultramicrotomy is a technique used in microscopy and material sciences to prepare very thin sections of specimens for transmission electron microscopy (TEM) or scanning electron microscopy (SEM). It involves cutting ultrathin sections (typically less than 100 nanometers thick) from a sample block. We maintain a diverse selection of diamond blades and other microtomy tools at DiATOME that are engineered to facilitate consistent, precise specimen preparations. More than this, we strive for innovative designs that level up the field and make it easier to achieve the highest-caliber results.

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Histology Sectioning

Histology Sectioning

Histology is the study of the microscopic structures of tissues and cells. In histology, tissue samples are embedded in paraffin or other embedding media, and then thin sections are cut for examination under a light microscope. This process is crucial for medical diagnostics and research, and requires the use of highly precise diamond blades for proper sectioning. We carry an array of histology tools and diamond blades for histology sectioning, including the DiATOME histo diamond knife. This tool is tailored for preparing semithin sections suitable for light microscopy, which requires thicker sections in comparison to TEM. The histo knife is used in a variety of light microscopy applications and fields, including biology, histology, pathology, and material sciences.

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Atomic Force Microscopy (AFM)

Atomic Force Microscopy (AFM) is a microscopy technique used for the detailed investigation of surface properties of materials and biological specimens at the nanoscale. In sample preparation for AFM, the exceptional sharpness and durability of diamond knives ensure that sections are of high quality and facilitate the preservation of surface properties during the sample preparation. The use of diamond knives and tools in AFM applications contributes to achieving accurate, reliable topographical data that enables researchers to gain valuable insights into the nanostructures and characteristics of specimens.

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Atomic Force Microscopy
Cryo Sectioning

Cryo Sectioning

Cryo sectioning is a sophisticated method used in the field of microscopy to meticulously prepare ultrathin frozen sections of biological specimens, including tissues, cells, or even entire organisms. By using diamond knives or tools in cryo sectioning, scientists ensure that the resulting ultrathin sections maintain the integrity of the biological specimens, allowing for high-resolution imaging and detailed structural analysis while keeping the samples as close to their natural state as possible.

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Resharpening Diamond Blades & Knives

Resharpening diamond knives is a crucial practice in microscopy applications, including TEM, SEM, and various other techniques. The resharpening process involves meticulously restoring the knife's cutting edge to its optimal sharpness. Our DiATOME diamond knives can be resharpened an unlimited amount of times without affecting the cutting-edge length. This durability is a result of our efficient resharpening process, which minimizes the amount of grinding during the sharpening procedure. Our unique process allows us to guarantee that your resharpened DiATOME knife will be of the same superior quality as your new DiATOME knife.

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Resharpening
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