cryo Diamond Knives

Cryo

DiATOME cryo knives and their applications

  • Thinner cryo sections
  • Perfect cryosections from ultrathin to semi with the same knife
  • Minimal compression and best structure preservation
  • Highest quality diamonds and optimal crystal orientation guarantee perfect ultrathin sections and a durable cutting edge
Knife type Knife angle Size
[mm]
Thickness
range [nm]
Boat type Code
(new knife)*
Application
cryo 25° 25°
3.0mm

30–150 Triangular holder 30-CD25 
  • Frozen hydrated samples (CEMOVIS)
cyro immuno 35° 2.0mm
3.0mm
30–300 Triangular holder DCIMM3520
DCIMM3530
  • Sucrose infiltrated samples (Tokuyasu)
  • Frozen hydrated samples (CEMOVIS)
cryo 35° (dry) 35°

1.5mm
2.0mm
2.5 mm
3.0 mm
3.5 mm
4.0 mm

30–300 Triangular holder 15-CDL
20-CDL
25-CDL
30-CDL
35-CDL
40-CDL
  • Polymers, rubber, paints etc.
cryo 35° (wet) 35° 1.5mm
2.0mm
2.5 mm
3.0 mm
3.5 mm
4.0 mm
30–300 Boat 15-CWL
20-CWL
25-CWL
30-CWL
35-CWL
40-CWL
  • Wet sectioning of polymers with DMSO/water mixture
cryo 45° (dry) 45° 1.5mm
2.0mm
2.5mm
3.0mm
3.5mm
4.0mm
30–300 Triangular holder 15-CDS
20-CDS
25-CDS
30-CDS
35-CDS
40-CDS
  • Routine dry cryo sectioning of polymers
cryo 45° (wet) 45° 1.5mm
2.0mm
2.5 mm
3.0 mm
3.5 mm
4.0 mm
30–300 Boat 15-CWS
20-CWS
25-CWS
30-CWS
35-CWS
40-CWS
  • Routine wet cryo sectioning of polymers with DMSO/water mixture
cryo AFM  35° 2.0mm
3.0mm
20–100 Triangular holder 30-20-AFM-CDL
30-AFM-CDL
  • Sample planing for AFM imaging

* Product Codes shown are for new knives.
To order a resharpened knife, add “R” to the Product Code that corresponds to your knife. 
Example: Product Code 30-CD25R = Resharpened cryo 25˚ 1.5mm Knife.

cryo 35˚ and 45˚ can be exchanged. To order an exchanged knife, add “E” to the Product Code.
Example: Product Code 15-CWLE = Exchange of an cryo 35˚ (wet) 1.5mm Knife

 

cryo 25°

The cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation (H.M. Han et al., Journal of Microscopy, Vol. 230, Pt. 2, pp. 167 – 171, 2007).

Please note: best results are achieved at low humidity, when the cryo-ultramicrotome is placed in a glovebox and the sections attached by electrostatic force (J. Pierson et al., Journal of Structural Biology 169, pp. 219 – 225, 2010).

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cryo 25

High resolution electron micrograph of vitreous section of keratin intermediate filaments in the midportion of stratum corneum of human epidermis. The fine structure of the keratin filaments is well resolved and their molecular organisation is seen in favourable cases (inset). Scale bar = 100 nm. Scale bar inset = 20 nm. Ashraf Al-Amoudi, Laboratoire d‘Analyse Ultrastructurale, Lausanne.


cryo immuno

The first cryo knife with a diamond platform, guarantees the best possible sectioning for sucrose infiltrated samples (Tokuyasu).

The diamond platform guarantees an easy and gentle section pick-up.

The sections are collected directly from the diamond surface using a loop and a sucrose/methyl-cellulose droplet (W. Liou et al., Histochemistry and Cell Biology, Vol. 106, pp. 41 – 55, 1996. P. J. Peters et al., Current Protocols in Cell Biology, pp. 4.7.1 – 4.7.18, 2006).

The 35° angle leads to a considerable reduction in mechanical stresses and therefore to improved structure preservation in sucrose infiltrated samples (E. Bos et al., Journal of Structural Biology 175, pp. 62 – 72, 2011).

We recommend the cryo immuno knife also for sectioning frozen hydrated samples (CEMOVIS). The 35° angle is a good compromise between durability and cutting performance (A. Al-Amoudi et al., Journal of Structural Biology 150, pp. 109 – 121, 2005).

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cryo immuno

Mouse optic nerve, immunolabeling of the major myelin protein proteolipid protein (PLP), 10 nm gold. Wiebke Möbius, Dept. of Neurogenetics, EM Core Facility, MPI of Experimental Medicine, Göttingen.


cryo 35° & cryo 45° (wet/dry)

The cryo 35° knife has demonstrated its usefulness as a standard knife for the low temperature sectioning of polymers, rubber, paints, etc.

The 35° angle represents a balanced compromise between section quality and durability.

The cryo 35° and cryo 45° knife mounted in the triangular holder is suitable for dry cryosectioning.

The cryo 35° and cryo 45° knife mounted in the trough are used for sectioning with fluids such as a DMSO/water mixture.

The cryo 45° knife is well suited for routine cryo sectioning.

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Shop cryo 35° (wet)

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cryo 35 and 45

Styrene-butadiene block copolymer x 25‘000 Ronald Walter, BASF Aktiengesellschaft, Polymer Physics, D-67056 Ludwigshafen.


cryo AFM

Our cryo AFM knives are of the highest quality to ensure the increased quality requirements of AFM investigation. They produce extremely smooth sample surfaces and guarantee the best possible structure preservation.

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cryo AFS

Top-view, light shaded AFM image of a cryomicrotomed surface of ultrahigh molecular weight polyethylene. The arrows indicate zones with lamellae splitting. P.H. Vallotton, Materials Science Division, Lawrence Berkley Laboratory. References Ref 1: P.H. Vallotton, M.M. Denn, B.A. Wood and M.B. Salmeron: Comparison of medical-grade ultrahigh molecular weight polyethylene microstructure by atomic force microscopy and transmission electron microscopy. J. Biomater. Sci. Polymer Edn., Vol 6, No. 7, 609-620, 1994. Top of Page

cryo AFS

Morphology of a blend of two SBS block copolymers with different chain-architecture. AFM tapping mode, phase image, image size = 3 x 3 μm. Rameshwar Adhikari, Institut für Werkstoffwissenschaft, Martin-Luther-Universität, Halle-Wittenberg.


Diamond Knives for CEMOVIS

For the cryo Electron Microscopist, we are pleased to present the DiATOME knives for sectioning vitrified cells and tissues.

Our application specialist is at your disposal for any further information or assistance you might require.

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Diamond Knives for CEMOVIS
Diamond Knives for CEMOVIS

26S proteosomes within cell nucleus of a Drosophila melanogaster neuron. M. Eltsov, IGBMC, Strasbourg, Scale bar: 50nm

Diamond Knives for CEMOVIS

Drosophila embryonic brain axons. Defocus -4µm, no phase plate Tomographic reconstruction, computational 5nm slice. M. Eltsov, IGBMC, Strasbourg Scale bar: 100nm