| Knife type |
Knife angle |
Size
[mm] |
Thickness
range [nm] |
Boat type |
Code
(new knife)* |
Application |
| cryo 25° |
25° |
3.0mm |
30–150 |
Triangular holder |
30-CD25 |
Frozen hydrated samples (CEMOVIS) |
The cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation (H.M. Han et al., Journal of Microscopy, Vol. 230, Pt. 2, pp. 167 – 171, 2007).
Please note: best results are achieved at low humidity, when the cryo-ultrami-crotome is placed in a glovebox and the sections attached by electrostatic force (J. Pierson et al., Journal of Structural Biology 169, pp. 219 – 225, 2010).
